Apr 05, 2024
Version 2
Tail Suspension Test V.2
- Marina Lorente Picón1,
- Núria Peñuelas1,
- Ariadna Laguna1,
- Marta González1,
- Miquel Vila1
- 1Vall d'Hebron Research Institute
- Vilalab Public
- Nuria
External link: https://doi.org/10.1038/s41467-024-53168-7
Protocol Citation: Marina Lorente Picón, Núria Peñuelas, Ariadna Laguna, Marta González, Miquel Vila 2024. Tail Suspension Test. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr3222vmk/v2Version created by Miquel Vila
Manuscript citation:
Laguna, A., Peñuelas, N., Gonzalez-Sepulveda, M. et al. Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related neurodegenerative deficits. Nat Commun 15, 8819 (2024). https://doi.org/10.1038/s41467-024-53168-7
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 05, 2024
Last Modified: April 08, 2024
Protocol Integer ID: 97833
Keywords: mice, test
Funders Acknowledgements:
Aligning Science Across Parkinson’s
Grant ID: ASAP-020505
Disclaimer
The protocols.io team notes that research involving animals and humans must be conducted according to internationally-accepted standards and should always have prior approval from an Institutional Ethics Committee or Board.
Abstract
Tail Suspension Test for mice
Troubleshooting
Suspend animals by their tails with tape in a suspension bar.
To avoid the tail climbing behavior, pass a 2cm methacrylate tube through the tail before suspending the animal.
Quantify the escape-oriented behaviors (i.e. fore and hind limbs movement) during six minutes. Vocalizations were registered during this period with a yes/no score.
Calculate the total immobilization time (s) as the sum of all the time the animal was not performing any escape-oriented behavior.