May 17, 2018
Tail-flick test
- 1Unifesp
Protocol Citation: Sunamita Lima 2018. Tail-flick test. protocols.io https://dx.doi.org/10.17504/protocols.io.p72drqe
Manuscript citation:
Pharmacological characterization of crotamine effects on mice hind limb paralysis employing both ex vivo and in vivo assays: insights into the involvement of voltage-gated ion channels in the crotamine action on skeletal muscles
Sunamita de Carvalho Lima1, Lucas de Carvalho Porta1, Álvaro da Costa Lima1, Joana D’Arc Campeiro1, Ywlliane Meurer2, Nathália Bernardes Teixeira3, Thiago Duarte1, Gisele Picolo3, Rosely Oliveira Godinho1, Regina Helena Silva1, Mirian Akemi Furuie Hayashi1, *
1Departamento de Farmacologia, Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil; 2Departamento de Fisiologia, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Brazil; 3Special Laboratory of Pain and Signaling, Instituto Butantan, São Paulo, Brazil.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 17, 2018
Last Modified: May 17, 2018
Protocol Integer ID: 12250
Mice are allowed to habituate for 30 min to the testing environment and the injection occur immediately the start of the test.
Each mouse is lightly restrained in a denim pocket while the distal half of it's tail is exposed into a thermostatically controlled temperature
Latency to respond to the heat stimulus by vigorous flexion of the tail is measured with a stopwatch.
Each mouse is given 3-5 trials with an inter-trial interval of 10 s, and a maximum trial duration of 8 s.
To prevent tissue injury each mouse's tail is promptly removed from the hot water after 30 s.
Latency performance of the last 3 trials are recorded.