Sep 09, 2020
T4H protocol
- Ophir Auslaender1,
- kalle.levon2
- 1NYU Grossman School of Medicine;
- 2NYU Tandon School of Engineering
- Tech4Health
Protocol Citation: Ophir Auslaender, kalle.levon 2020. T4H protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bk5iky4e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 08, 2020
Last Modified: September 09, 2020
Protocol Integer ID: 41866
Clean Au chip: insert in piranha solution (75% H2SO4, 25% H2O2), wash with DI water, dry with nitrogen gas.
Mix the solution for imprinting in 19/1 volume ratio: 19 for the analyte, 1 for the monolayer former at self-assembled monolayer (SAM) concentration.
Place Au chip in the imprinting solution.
Incubate overnigth RT.
Wash multiple times with 3M NaCl solution.
Leave overnight in DI water.
At this point the chip can be sealed for future use.
Prior to measurement chip is inserted in PBS for 2 hours.
The chip is connected as the working electrode in a potentiostat circuit.
A reference electrode is added to the reaction vessel and recording of the open-circuit potential (OCP) is started.
When the voltage stablilizes, the analyte is added.
Adding the analyte shifts the OCP if the analyte is recognized by the imprinted working electrode.
In the calibration phase, controlled serial dilutions of analyte are added, and resulting OCP values are recorded. The relationship between analyte concenteration and OCP is established from these calibration measurements, and is used for the detection of unknown analytes.