Jun 09, 2020
Türk solution and Trypan Blue
This document is a draft, published without a DOI.
- Marco Cosentino1,
- Elisa Storelli1,
- Alessandra Luini1,
- Massimiliano LM Legnaro1,
- Emanuela Rasini1,
- Marco Ferrari1,
- Franca Marino1
- 1Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy)
Document Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano LM Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. Türk solution and Trypan Blue. protocols.io https://protocols.io/view/t-rk-solution-and-trypan-blue-bg36jyre
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: June 03, 2020
Last Modified: June 09, 2020
Document Integer ID: 37726
Abstract
This recepe is used in the following protocols:
- Separation and purification of human PBMC from FRESH BLOOD
- Separation and purification of human PBMC from BUFFY COAT
- Magnetic bead-based CD4+ T cell isolation from PBMCs with Dynabeads: CD4 Positive Isolation Kit
- Magnetic bead-based TREG-TEFF cell isolation from PBMC with Miltenyi CD4+CD25+ Regulatory T cell Isolation Kit
- Separation of Human Neutrophils (PMN) from Buffy Coat
- Separation of Human Neutrophils (PMN) from Whole Blood
Türk solution composition (v/v):
- Gentian Violet 0.02%
- Acetic Acid 3% prepared in H2O.
Storage:4°C in the dark covered with aluminum foil (photosensitive)
Trypan Blue solution 0.4%
Prepare a dilution at 0.2%(v/v): 0.4 mL of Trypan stock solution + 0.6 mL PBS or NaCl (final volume 1mL).
Storage:4°C in the dark (Prepare fresh every 2 weeks)
Gentian violet: Marco Viti
Acetic acid catalog number: A6283 Sigma Italy
Trypan blue catalog number: T8154-100mL Sigma Italy