License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: June 18, 2026
Last Modified: June 24, 2026
Collection Integer ID: 319385
Keywords: non-human primate, Surgical procedure, Chamber implantation, Craniotomy, Chamber maintenance, stable cranial interface, stabilization hardware to the skull, craniotomy, human primates this collection, human primate, surgical preparation, injection chamber, detailed surgical procedure, skull, step methodological framework for the surgical preparation, chronic implant, term maintenance of chronic recording, chamber maintenance protocol, lifespan of the chronic implant, chronic attachment of the recording, head, chamber maintenance
Funders Acknowledgements:
NINDS
Grant ID: Grant ID: R01NS129551
NINDS
Grant ID: Grant ID: R21NS101499
The Brain Sciences Project of the CNSI & NINS Grant
Grant ID: Grant ID: BS291006
Disclaimer
These protocols are for scientific research purposes only and must be conducted under the approval of the appropriate Institutional Animal Care and Use Committee (IACUC). Implementation requires specialized surgical training and veterinary expertise. The authors assume no liability for any complications or experimental failures resulting from the use of these procedures.
Abstract
This collection provides a comprehensive, step-by-step methodological framework for the surgical preparation and long-term maintenance of chronic recording and injection chambers in behaving non-human primates (NHPs). Establishing and maintaining a stable cranial interface is critical for executing reproducible experiments over extended periods.
This collection integrates three interconnected, high-resolution protocols:
Chamber and Head-Post Implantation: Detailed surgical procedures for the secure, chronic attachment of the recording/injection chamber and head-stabilization hardware to the skull.
Craniotomy: Precise guidelines for performing the craniotomy.
Chamber Maintenance (Cleaning and Dura Scraping): Daily and long-term post-operative care routines.
These protocols are designed to maximize animal welfare, extend the lifespan of the chronic implant, and ensure high experimental reproducibility across subjects.
Before start
This protocol requires prior approval from the users' Institutional Animal Care and Use Committee (IACUC) or an equivalent ethics committee.
Disclaimer
These protocols are for scientific research purposes only and must be conducted under the approval of the appropriate Institutional Animal Care and Use Committee (IACUC). Implementation requires specialized surgical training and veterinary expertise. The authors assume no liability for any complications or experimental failures resulting from the use of these procedures.
