Mar 06, 2020
- 1Hainan University
External link: https://doi.org/10.1371/journal.pone.0234435
Protocol Citation: Jing Xu 2020. Superoxide Radical-scavenging Activity. protocols.io https://dx.doi.org/10.17504/protocols.io.bdaxi2fn
Manuscript citation:
Zhou J, Yang Q, Zhu X, Lin T, Hao D, Xu J (2020) Antioxidant activities of Clerodendrum cyrtophyllum Turcz leaf extracts and their major components. PLoS ONE 15(6): e0234435. doi: 10.1371/journal.pone.0234435
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 06, 2020
Last Modified: March 06, 2020
Protocol Integer ID: 33847
Abstract
The superoxide radical scavenging effects were examined [11]. Briefly, extract (1 ml) was added to 1 ml of 50 μM NBT solution and 1 ml of 468 μMNADH, and a 1ml aliquot of 60 μM PMS reaction mixture. After 5min, absorbance was read at 560 nm. The inhibition percent and IC50 valueswere calculated.