Apr 19, 2020
Version 2
Successful and cost-effective DNA extraction method from insects with small amount of tissue V.2
- 1Sharjah Research Academy
Protocol Citation: Rahul Jamdade 2020. Successful and cost-effective DNA extraction method from insects with small amount of tissue . protocols.io https://dx.doi.org/10.17504/protocols.io.bfajjicn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 19, 2020
Last Modified: April 19, 2020
Protocol Integer ID: 35883
Abstract
DNA extraction from insects is an initial and critical step that might affect the molecular taxonomy. There are several protocols for efficient DNA extraction published by researchers, which might be helpful for others working in the relevant field. There are also some commercial kits optimized to extract DNA even from low volumes or less amount of tissue. Research has conventionally showed that Phenol: chloroform: Isoamyl alcohol (PCI) method gives good yield and is cost effective as compared to other methodologies, especially commercial kits. Hereby is the CTAB and PCI protocol, which gives good yield from insect tissue preferably legs, head and wings (avoid using whole body).
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