The following protocol describes the acoustic droplet ejection dispenses needed for a minitaturized 16S PCR reaction using the Echo 550. The protocol expects (1) 384-Well 16S Illumina Primer Plate in an echo qualified Low Dead Volume (LDV) plate, (1) 384-Well Extracted gDNA in an echo qualified Polypropylene (PP) plate, and (3) 384-Well PCR Plates in twin.tec PCR plates.