Mar 04, 2025
- Katerina Rademacher1,
- Ken Nakamura1
- 1Gladstone Institutes
External link: https://doi.org/10.7554/eLife.98775
Protocol Citation: Katerina Rademacher, Ken Nakamura 2025. Striatal Punch Collection. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp97xdvzp/v1
Manuscript citation:
Rademacher K, Doric Z, Haddad D, Mamaligas A, Liao S, Creed R, Kano K, Chatterton Z, Fu Y, Garcia JH, Vance VM, Sei YJ, Kreitzer A, Halliday G, Nelson AB, Margolis E, Nakamura K Chronic hyperactivation of midbrain dopamine neurons causes preferential dopamine neuron degeneration. eLife 13(). doi: 10.7554/eLife.98775
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 27, 2025
Last Modified: March 05, 2025
Protocol Integer ID: 123561
Keywords: ASAPCRN, striatal punches from fresh frozen mouse brain tissue, striatal punch collection this protocol, striatal punch collection, striatal punch, fresh frozen mouse brain tissue, hplc analysis, mouse, brain, tissue
Funders Acknowledgements:
ASAP
Grant ID: 025184
Abstract
This protocol describes how to collect striatal punches from fresh frozen mouse brain tissue for HPLC analysis.
Troubleshooting
Striatal Punch Collection
Sacrifice mice with CO2, rapidly remove the brain, and freeze in superchilled isopentane on dry ice. Store brains at -80˚C until ready to collect tissue.
Embed brains in OCT, and mount on a cryostat. Trim until the striatum, then take punches from the dorsal striatum using a pipette tip cut to 1mm in diameter.
Store striatal punches at -80˚C until ready to ship for analysis. Ship on dry ice.