Jan 23, 2026
Version 8
Stereotaxic Injection by Nanoject Protocol V.8
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain Science
- Allen Institute for Neural Dynamics
Protocol Citation: Allen Institute for Brain Science 2026. Stereotaxic Injection by Nanoject Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6nr7kgqe/v8Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 23, 2026
Last Modified: February 03, 2026
Protocol Integer ID: 240830
Keywords: Surgery, tracers, injection, nanoject, stereotaxic injection by nanoject protocol, stereotaxic injection, nanoject ii, specific brain coordinates in the mouse, nanoject protocol, neuronal tracer, stereotaxic system, specific brain coordinate, mouse, national institutes of health
Abstract
This protocol describes the delivery of a neuronal tracer using the Nanoject II. The surgery uses a stereotaxic system to target specific brain coordinates in the mouse.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
