Dec 19, 2025
Version 8
Stereotaxic Injection by Iontophoresis V.8
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2025. Stereotaxic Injection by Iontophoresis. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn8ewzg5d/v8Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 19, 2025
Last Modified: December 22, 2025
Protocol Integer ID: 235510
Keywords: AF0092, surgery, tracers, injection, iontophoresis, stereotaxic injection by iontophoresi, stereotaxic injection, iontophoresi, iontophoretic method, neuronal tracer, specific brain coordinates in the mouse, stereotaxic system, specific brain coordinate, mouse,
Abstract
This protocol describes the delivery of a neuronal tracer using the iontophoretic method. The surgery uses a stereotaxic system to target specific brain coordinates in the mouse.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
