May 05, 2020
Starting a MinION sequencing run using MinKNOW
Forked from Starting a MinION sequencing run using MinKNOW
- 1Massey University
- Coronavirus Method Development Community
Protocol Citation: Muhammad Faisal, Olin Silander, Nikki Freed 2020. Starting a MinION sequencing run using MinKNOW. protocols.io https://dx.doi.org/10.17504/protocols.io.bfwqjpdw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: May 02, 2020
Last Modified: May 05, 2020
Protocol Integer ID: 36528
If required plug the MinION into the computer and wait for the MinION and flowcell to ben detected.
Choose flow cell 'FLO-MIN106' from the drop-down menu.
Then select the flowcell so a tick appears.
Click the 'New Experiment' button in the bottom left of the screen.
On the New experiment popup screen, select the running parameters for your experiment from the individual tabs:
Experiment: Name the run in the experiment field, leave the sample field blank.
Kit: Selection: Select RBK004
Run Options: Set the run length to 6 hours (you can stop the run once sufficient data has been collected as determined using RAMPART).
Basecalling: Select 'fast basecalling'.
Output: The number of files that MinKNOW will write to a single folder. By default this is set to 4000 but can be reduced to make RAMPART update more frequently.
Click 'Start run'.
Monitor the progress of the run using the MinKNOW interface.