Standard hematological analyses EDTA-anticoagulated blood was used for cytometric analysis using a whole blood counter Sysmex XE 2100® (Sysmex,Kobe, Japan) to obtain a whole blood count. Fibrinogen levels were determined with an ACL-9000 (Diamond Diagnostics, Holliston, MA) coagulation analyser, using a PT-fibrinogen high sensitivity reagent, which is a high-sensitivity calcium thromboplastin that allows the simultaneous determination of PT and fibrinogen levels. aPTT was measured using an ACL-9000 coagulation analyser and INR was calculated by the formula INR (PT patient/PT normal).