Sep 11, 2024
Version 1
Spray inoculation of peach trees or leaves with the plant pathogen Xanthomonas arboricola pv. pruni. V.1
- Daniela Negrete-Moreno1,
- Annabel Miller1,
- Elizabeth Cieniewicz1
- 1Clemson University
Protocol Citation: Daniela Negrete-Moreno, Annabel Miller, Elizabeth Cieniewicz 2024. Spray inoculation of peach trees or leaves with the plant pathogen Xanthomonas arboricola pv. pruni.. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lywbxrvx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 11, 2024
Last Modified: September 11, 2024
Protocol Integer ID: 107371
Keywords: Spray-inoculations, Xap, detached leaf assays, and potted tree assays, peach leaves with the bacterium, spray inoculation of peach tree, viruses in peach, inoculations of potted peach tree, multiple pathogen interaction, potted peach tree, plant pathogen xanthomonas arboricola pv, effects of multiple pathogen interaction, leaves with the plant pathogen xanthomonas arboricola pv, pathogen, detached peach leaf, leaf assay, detached leaf assay, peach tree, potted tree experiment, single pathogen, host cultivar, leading peach, tolerance of different cultivar, peach, orchard, different cultivar, bacterial colony, bacterium, multiple pest, specific spray inoculation method, spray inoculation, defense response, xanthomonas arboricola, virus
Abstract
Description:
Potted tree experiments and detached leaf assays are used for many purposes, including:
- observing the effects of a single pathogen on a host cultivar,
- observing the effects of multiple pathogen interactions on a host cultivar
- comparing the defense responses and tolerance of different cultivars to pathogen attack.
Abstract:
South Carolina is the leading peach-producing state in the southeastern United States, where multiple pests and pathogens regularly threaten orchards. Here, we describe a methodology for controlled inoculations of potted peach trees or detached peach leaves with the bacterium Xanthomonas arboricola pv. pruni (Xap). This protocol is used primarily to study interactions between Xap and viruses in peach. To further understand the effects of a bacterial-viral co-infection, detached leaf assays serve as a controlled representation of what happens in the field. Hence, we report developing a Xap-specific spray inoculation method that inoculates a medium directly from bacterial colonies.
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Protocol references
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