Feb 12, 2020
SPARC Segmentation Conversion: Binary mask tracings to MBF XML
- 1MBF Bioscience, MAP-CORE
- SPARCTech. support email: info@neuinfo.org
Protocol Citation: Maci Heal, Susan Tappan 2020. SPARC Segmentation Conversion: Binary mask tracings to MBF XML. protocols.io https://dx.doi.org/10.17504/protocols.io.bchjit4n
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 12, 2020
Last Modified: February 12, 2020
Protocol Integer ID: 33035
Abstract
Convert binary mask tracings from Nikon's NIS Elements software into MBF XML format.
Materials
MATERIALS
Neurolucida 360 SoftwareMBF Bioscience
Conversion
Conversion
Launch Neurolucida 360.
Open binary mask tracings (.tif) into program window.
Add subject metadata and select criteria for anatomic term list in the SPARC Vocabulary Services welcome dialog.
Choose the approrpiate vocabulary term from the contour selection toolbar.
Select the Outline Objects icon from the Trace ribbon.
Click on a black or white pixel on the binary mask tracings to automatically contour the representative object.
Repeat until all objects are contoured.
Save file as XML.