This protocol is used for acute surgery and electrophysiological experimentation on the anesthetized ferret, specifically for manipulating the vagus nerve and gastrointestinal tract nerve stimulation and myoelectric recording.Funding: This protocol was developed with funding from the NIH Common Fund’s Stimulating Peripheral Activity to Relieve Conditions (SPARC) program (Award U18TR002205). To learn more about the SPARC program, visit https://sparc.science.