Nov 21, 2020
SOLUTION- 13 - Complete culture medium
- Marco Cosentino1,
- Elisa Storelli1,
- Alessandra Luini1,
- Massimiliano LM Legnaro1,
- Emanuela Rasini1,
- Marco Ferrari1,
- Franca Marino1
- 1Center for Research in Medical Pharmacology, University of Insubria (Varese)
Document Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano LM Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. SOLUTION- 13 - Complete culture medium. protocols.io https://dx.doi.org/10.17504/protocols.io.bpxkmpkw
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: November 21, 2020
Last Modified: November 21, 2020
Document Integer ID: 44748
Prepare 50 mL culture medium (RPMI/FBS 10%/ Pen-Strep 100 U/mL) with:
44,5 mL RPMI 1640 with phenol-red supplemented with 5 mL of FBS (Fetal Bovine Serum) and 0,5 mL penicillin/streptomicin (equivament of 100 U/mL).
Alle reagents can be found in Fridge 2 - (Room PSO3). Work under a laminar flow hood (Room PSO3).
Storage: 4 °C Fridge 2 - (Room PSO3).
RPMI: catalog number ECM0495L, Euroclone, Italy.
FBS: catalog number ECS0180L, Euroclone, Italy.
Penicillin/Streptomycin: Catalog number ECB3001D - 100 ml, Euroclone, Italy.