Jul 31, 2020
SOLUTION- 11 - CD4+T cells isolation buffer
- Marco Cosentino1,
- Elisa Storelli1,
- Alessandra Luini1,
- Massimiliano LM Legnaro1,
- Emanuela Rasini1,
- Marco Ferrari1,
- Franca Marino1
- 1Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy)
Document Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano LM Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. SOLUTION- 11 - CD4+T cells isolation buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.bi79khr6
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: July 31, 2020
Last Modified: July 31, 2020
Document Integer ID: 39905
Composition g/ml:
BSA 0.250 g
EDTA 0.2235 g
Add PBS(Ca2+ and Mg2+ free) to 240 ml, adjust to pH7.4 with NaOH or HCl, bring to 250 ml with PBS.
The substances are in the closet 1 and in refrigerator 1 (Room TSO8), weigh the substances with Gibertini balance (Room TSO8).
Filter this buffer by using a sterile syringe and filter.
Sterile filters and syringes are in closet 1 (Room PSO3).
Storage:4 °C refrigerator 2 - (Room PSO3).
BSA: catalog number A2153 , Sigma, Italy
EDTA: catalog number ED2SS, Sigma, Italy
Prymo siringe filter: catalog number EPSPE2230 Euroclone, Italy.