Aug 03, 2020
SOLUTION- 10 - TEFF/TREG isolation buffer
- Marco Cosentino1,
- Elisa Storelli1,
- Alessandra Luini1,
- Massimiliano LM Legnaro1,
- Emanuela Rasini1,
- Marco Ferrari1,
- Franca Marino1
- 1Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy)
Document Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano LM Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. SOLUTION- 10 - TEFF/TREG isolation buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.bjadkia6
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: August 03, 2020
Last Modified: August 03, 2020
Document Integer ID: 39973
This buffer is composed of PBS, 0.5% BSA and 2mM EDTA.
It must be cold (2-8°C) and sterile filtered before use.
BSA 1.250 g,
EDTA 0.2235 g
Dissolve the following powders in 240 mL PBS 1X, adjust the pH to 7.2 with HCl or NaOH, and then add PBS 1X to 250 mL.
Storage:4 °C (Fridge - 2 Room PS03)
BSA: catalog number A2153 , Sigma, Italy
EDTA: catalog number ED2SS, Sigma, Italy