Nov 21, 2020

Public workspaceSOLUTION- 02 - Phosphate Buffered Saline (PBS)

DOI
https://dx.doi.org/10.17504/protocols.io.bpximpke
  • Marco Cosentino1,
  • Elisa Storelli1,
  • Alessandra Luini1,
  • Massimiliano Legnaro1,
  • Emanuela Rasini1,
  • Marco Ferrari1,
  • Franca Marino1
  • 1Center for Research in Medical Pharmacology, University of Insubria (Varese)
Farmacologia Medica
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DOI: https://dx.doi.org/10.17504/protocols.io.bpximpke
Document Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. SOLUTION- 02 - Phosphate Buffered Saline (PBS). protocols.io https://dx.doi.org/10.17504/protocols.io.bpximpke
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: November 21, 2020
Last Modified: November 21, 2020
Document Integer ID: 44746
Keywords: purification of human pbmc, teff cell isolation from pbmc, human pbmc, teff cell isolation, flow cytometry, pbmc, cell proliferation evaluation, phosphate buffered saline, cd4 positive isolation kit, isolated subsets with cell proliferation dye, dynabeads cd4 positive isolation kit, cell proliferation dye, fresh blood
Abstract
This recepe is used in the following protocols:
- Separation and purification of human PBMC from FRESH BLOOD
- Separation and purification of human PBMC from BUFFY COAT
- Magnetic bead-based CD4+ T cell isolation from PBMCs with Dynabeads: CD4 Positive Isolation Kit
- Magnetic bead-based TREG-TEFF cell isolation from PBMC with Miltenyi CD4+CD25+ Regulatory T cell Isolation Kit
- Staining of human PBMC or ISOLATED SUBSETS with Cell Proliferation Dye-eFluor™ 670 (CPD-eFluor670) for cell proliferation evaluation by Flow Cytometry
Troubleshooting
Phosphate-buffered saline (PSB 1X)

NaCl- 8 g (137 mM)
KCl- 1.5 g (2 mM)
Na2HPO4- 1.44 g (10 mM)
KH2PO4- 0.24 g (1.8 mM)

To prepare 1 L of PBS 1X, dissolve the reagents listed above in 800 mL of ultrapure H2O.
Adjust the pH to 7.4 with HCl or NaOH, and then add H2O to 1 L.

NaClcode: S9625, Sigma
KClcode: P9541, Sigma
Na2HPO4code: 1.06585, Sigma
KH2PO4code: P0662, Sigma