Jul 23, 2020

Public workspaceSOLUTION- 02 - Phosphate Buffered Saline (PBS) V.2

DOI
dx.doi.org/10.17504/protocols.io.biahkab6
  • 1Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy)
Elisa Storelli
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DOI: dx.doi.org/10.17504/protocols.io.biahkab6
Document Citation: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano LM Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. SOLUTION- 02 - Phosphate Buffered Saline (PBS). protocols.io https://dx.doi.org/10.17504/protocols.io.biahkab6
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: July 07, 2020
Last Modified: July 27, 2020
Document Integer ID: 38953
Abstract
This recepe is used in the following protocols:

- Separation and purification of human PBMC from FRESH BLOOD
- Separation and purification of human PBMC from BUFFY COAT
- Magnetic bead-based CD4+ T cell isolation from PBMCs with Dynabeads: CD4 Positive Isolation Kit
- Magnetic bead-based TREG-TEFF cell isolation from PBMC with Miltenyi CD4+CD25+ Regulatory T cell Isolation Kit
- Staining of human PBMC or ISOLATED SUBSETS with Cell Proliferation Dye-eFluor™ 670 (CPD-eFluor670) for cell proliferation evaluation by Flow Cytometry



Phosphate-buffered saline (PSB 1X)

NaCl - 8 g (137 mM)
KCl - 0.2 g (2.7 mM)
Na2HPO4 - 1.44 g (10 mM)
KH2PO4 - 0.24 g (1.8 mM)

To prepare 1 L of PBS 1X, dissolve the reagents listed above in 800 mL of ultrapure H2O.
Adjust the pH to 7.4 with HCl or NaOH, and then add H2O to 1 L.


NaCl code: S9625, Sigma
KCl code: P9541, Sigma
Na2HPO4 code: 1.06585, Sigma
KH2PO4 code: P0662, Sigma

Fonte: (Cold Spring Harbor Laboratory Press 2006)