Jun 11, 2026

Solid medium fungal culture extraction

DOI
https://dx.doi.org/10.17504/protocols.io.5jyl83np9v2w/v1
  • Clémence ROHÉE1,
  • Alice M.S. Rodrigues1,
  • Yoan Ferandin1,
  • Emeline HOUEL1,
  • Didier STIEN1
  • 1Sorbonne Université, CNRS, UPVD, UMR8176, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, 66650 Banyuls-sur-Mer, France.
  • EUREMAP PROTOCOLS COMMUNITY
  • EUREMAP_SU_LBBM
Clémence ROHÉE
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DOI: https://dx.doi.org/10.17504/protocols.io.5jyl83np9v2w/v1
Protocol Citation: Clémence ROHÉE, Alice M.S. Rodrigues, Yoan Ferandin, Emeline HOUEL, Didier STIEN 2026. Solid medium fungal culture extraction. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl83np9v2w/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 08, 2026
Last Modified: June 11, 2026
Protocol  Integer ID: 318676
Keywords: solid medium fungal culture extraction this protocol, solid medium fungal culture extraction, fungal culture dry extract in solid medium, fungal culture, dry extract, biological testing
Funders Acknowledgements:
European Union
Grant ID: INFRA-2023-DEV-01-04
Abstract
This protocol describes how to obtain a fungal culture dry extract in solid medium for biological testing or LC-MS analysis.
Materials
HPLC- grade Methyl Ethyl Ketone (MEK)
250mL glass bottle with screw cap
Scalpel and blades
Funnel
Decanting funnel
Round-bottomed flask
Rotary evaporator
Pastor pipettes
5mL glass tubes
Evaporator (centrifugal)
-20 °C freezer
Safety warnings
Use nitrile gloves, safety goggles and lab coat.
Maceration preparation
Fungi cultures are grown in 14.5 cm diameter Petri dishes, which hold 50 mL of culture medium.
Using a scalpel, squares measuring approximately 1 cm² are cut out and transferred to a 250 mL glass bottle.
50 mL of MEK are added and the bottle is closed.
The bottle is placed on a rotary shaker at room temperature for approximately 20 hours (120 rpm).

Obtaining the extract
On the following day, the bottle content is poured into a decanting funnel fitted with a funnel to prevent the transfer of agar pieces.
The liquid aqueous phase formed during maceration is discarded and the organic phase, on top, is transferred to a round-bottomed flask. (dMEK=0.8)
The organic phase is evaporated to dryness with a rotary evaporator.
The extract is then taken up with few mL of MEK and transferred in a weighed 5 mL-glass tube with a Pastor pipette.
The tube is evaporated to dryness with a centrifugal evaporator.
The extract is weighed and preserved at -20°C.