Sep 22, 2019

Public workspaceSOC Medium

DOI
dx.doi.org/10.17504/protocols.io.7juhknw
  • 1Wageningen University
  • iGEM Wageningen 2019
Alba Balletbó
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.7juhknw
Protocol Citation: Alba Balletbó 2019. SOC Medium. protocols.io https://dx.doi.org/10.17504/protocols.io.7juhknw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 22, 2019
Last Modified: September 22, 2019
Protocol Integer ID: 27988
Guidelines
Mixture must be autoclaved before use.
Materials
MATERIALS
ReagentMagnesium Sulfate (MgSO4) Solution - 6.0 mlNew England BiolabsCatalog #B1003S
ReagentDistilled Water
ReagentKCl
ReagentMgCl2
ReagentNaClMerck MilliporeSigma (Sigma-Aldrich)Catalog #53014
ReagentTryptoneFisher ScientificCatalog #BP1421-500
ReagentGlucoseMerck MilliporeSigma (Sigma-Aldrich)Catalog #G8270
ReagentYeast ExtractThermo FisherCatalog #211930
Add SOC medium components in a 1 L autoclavable. SOC medium components (1 L):

Tryptone2% w/v
Yeast Extract0.5% w/v
NaCl10 mM
KCl2.5 mM
MgCl2 · 6H2O10 mM
MgSO4 · 6H2O10 mM
Glucose20 mM


Add 1 L of distilled water into the bottle.
Vigorously shake the bottle.
Place the bottle into the autoclave and sterilize.