Jun 23, 2026
Version 6
SMARTerV4 (0.5x) Amplification for single-cell or single-nuclei RNASeq Protocol V.6
- Allen Institute1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute 2026. SMARTerV4 (0.5x) Amplification for single-cell or single-nuclei RNASeq Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv517xdl1b/v6Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 23, 2026
Last Modified: June 23, 2026
Protocol Integer ID: 319701
Keywords: SMARTerV4 (0.5x), SMARTerV4, nuclei rnaseq protocol protocol, length cdna from single cell, using takara smarter v4, takara smarter v4, smarterv4, length cdna, single cell, nuclei, amplification, cell
Abstract
Protocol to generate full-length cDNA from single cells, or nuclei, using Takara SMARTer V4.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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