Nov 29, 2023

SM buffer for making phage dilutions

DOI
https://dx.doi.org/10.17504/protocols.io.bp2l6xykrlqe/v1
  • 1Macquarie University;
  • 2jaschke labs
theo.casalibain TCB Casali-Bain
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DOI: https://dx.doi.org/10.17504/protocols.io.bp2l6xykrlqe/v1
Protocol Citation: HANNAH ZHU 2023. SM buffer for making phage dilutions. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6xykrlqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 29, 2023
Last Modified: November 29, 2023
Protocol Integer ID: 91596
Keywords: making phage dilution, phage dilution, sm buffer, dilution
Abstract
How to make sm buffer for phage dilutions
Dissolve following components in 300 mL of MilliQ water:



AFinal concentrationFor 400 mL
NaCl100 mM2.34 g
Tris base50 mM2 g
MgSO 4 •7H 2 O8 mM0.79 g

Adjust pH to 7.4 – 7.5 with HCl

Top up the volume to 400 mL with MilliQ water

Autoclave at 121 °C for 00:15:00

15m
Cool down to room temperature
Filter-sterilise through 0.22 µm membrane
Store at room temperature