Sirius Red staining is a usefull staining to study collagen distribution. In adipose tissue development during obesity the extracellular matrix is being remodeled and this can be analyzed using Sirius Red staining. In addition, fibrosis in livers from mice suffering from non-alcoholic steatohepatitis can be identified using this staining. Moreover, scar formation after the induction of a myocardial infarct can be illustrated using the Sirius Red staining.