Oct 18, 2020

Public workspaceSingle digested

DOI
dx.doi.org/10.17504/protocols.io.bni5mcg6
  • 1South China University of Technology
Jiaxin Li
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DOI: dx.doi.org/10.17504/protocols.io.bni5mcg6
Protocol Citation: Jiaxin Li 2020. Single digested. protocols.io https://dx.doi.org/10.17504/protocols.io.bni5mcg6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 18, 2020
Last Modified: October 18, 2020
Protocol Integer ID: 43325
Add the following reagents to a tube.

Total10ul
10 x Buffer1ul
Template（500ng）According to different template
Enzyme0.5ul
ddWaterAdd to 10ul
Pipette up and down thoroughly.
Put at 37℃ for about 1.5 hours (according to different enzymes)