Previously, RNA sequencing for whole-genome gene expression analysis could only be performed on whole tissue (bulk RNA seq), or microdissected tissue compartments, where gene expression measurements reflect an average across all captured cell types. Single cell RNA sequencing allows the measurement and comparison of gene expression of individual cells and thus capture the previously underappreciated cellular heterogeneity within tissues such as the kidney. Our protocol allows the dissociation of a single kidney biopsy core to a single cell suspension. The total mRNA from each living cell is then captured, uniquely barcoded, and gene expression profiled with RNAseq. Single cell transcriptomics of adult kidney tissue is performed using 10X Genomics droplet-based technology. 10X Genomics technology combines microfluidics with molecular barcoding and custom bioinformatics software (CellRanger). High-throughput single cell transcriptomic measurements enable profiling of individual cell types.