Dec 23, 2025
Single-cell extracellular recordings Surgery
- Asa Mackenzie1
- 1Lund University
Protocol Citation: Asa Mackenzie 2025. Single-cell extracellular recordings Surgery. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk1541g5r/v1
Manuscript citation:
Serra GP, Guillaumin A, Vlcek B, Delgado-Zabalza L, Ricci A, Rubino E, Dumas S, Baufreton J, Georges F, Wallén-Mackenzie Å. A role for the subthalamic nucleus in aversive learning. Cell Rep. 2023 Nov 28;42(11):113328. doi: 10.1016/j.celrep.2023.113328. Epub 2023 Nov 4. PMID: 37925641.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 23, 2025
Last Modified: December 23, 2025
Protocol Integer ID: 235673
Keywords: ASAPCRN, cell extracellular recordings surgery stereotaxic virus injection, extracellular recordings surgery stereotaxic virus injection, stereotaxic virus injection, fiber optic probe implantationsingle, cell
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-020600
Abstract
Stereotaxic virus injection and fiber optic probe implantationSingle-cell extracellular recordings
Surgery
Troubleshooting
Single-cell extracellular recordings Surgery
In vivo single cell extracellular recordings started after a post-injection recovery period of at least 4 weeks.
Mice were anesthetized with a mix isoflurane-air (0.8–1.8% v/v) and placed in a stereotaxic apparatus.
Optic fiber, optrode and glass micropipettes coordinates were AP = 1.90 mm, ML = +/ 1.70 mm and DV = 4.30 mm for the STN and AP = 1.60 mm, ML = +/0.50 mm, DV = 2.00
to 3.30 mm for the LHb.