Feb 06, 2026
Setup and Operation of PX2+ Photometric Transmitter With Integrated Fluorescence Probe for Cell Culture
This protocol is a draft, published without a DOI.
- Ugo Okwuadigbo1
- 1MIT
Protocol Citation: Ugo Okwuadigbo 2026. Setup and Operation of PX2+ Photometric Transmitter With Integrated Fluorescence Probe for Cell Culture. protocols.io https://protocols.io/view/setup-and-operation-of-px2-photometric-transmitter-hrd5b5287
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: February 06, 2026
Last Modified: February 06, 2026
Protocol Integer ID: 242845
Keywords: photometric transmitter with integrated fluorescence probe, real time fluorescence measurement, fluorescence probe for real time fluorescence measurement, reproducible fluorescence measurements across user, reproducible fluorescence measurement, integrated fluorescence probe, photometric transmitter, operation of px2, operation of the px2, px2, cell culture this protocol, standalone operation for rapid measurement, cell culture, data logging, control panel app for data logging
Abstract
This protocol describes the setup and operation of the PX2+ Photometric Transmitter with an integrated fluorescence probe for real time fluorescence measurement and optional data logging. Two operating modes are described: use with the PX2+ Control Panel App for data logging and calibration, and standalone operation for rapid measurements without data collection. This protocol is intended for reproducible fluorescence measurements across users and experimental sessions.
Materials
PX2+ Photometric Transmitter
Integrated fluorescence probe
Power supply compatible with PX2+
USB cable
Computer with PX2+ Control Panel App installed
USB flash drive containing PX2+ Control Panel App
Troubleshooting
Problem
No signal is observed
Solution
Confirm that the fluorescence probe is securely connected.
Problem
The device is not detected by the Control Panel App
Solution
Verify the USB connection and restart the application.
Before start
Ensure that all components are present and undamaged.
Confirm that the PX2+ Control Panel App is available on the provided USB flash drive or already installed on the computer.
Determine whether the experiment requires data logging or only real time fluorescence visualization.
Operation With PX2+ Control Panel App
Install the PX2+ Control Panel App on the computer using the provided USB flash drive.
Connect the integrated fluorescence probe to the PX2+ Photometric Transmitter.
Connect the PX2+ Photometric Transmitter to the computer using the USB cable.
Supply power to the PX2+ Photometric Transmitter only after the USB connection has been established.
Launch the PX2+ Control Panel App on the computer.
Verify that the device is recognized by the software interface.
Enable data logging by navigating to Tools, then Logging Setup, then Status, and selecting Enable Logging.
Select a file location for data storage. Fluorescence data will be recorded and saved as a CSV file.
Confirm that fluorescence signal values are updating in real time within the application interface.
Standalone Operation Without PX2+ Control Panel AppUntitled section
Ensure that the PX2+ Photometric Transmitter is not connected to a computer.
Connect the integrated fluorescence probe to the PX2+ Photometric Transmitter.
Supply power to the PX2+ Photometric Transmitter.
Observe real time fluorescence output via the device interface.