Mar 17, 2022
Version 2
Serotyping V.2
- 1Universidad Autonoma de Nuevo Leon
External link: https://doi.org/10.1371/journal.pone.0264273
Protocol Citation: Lydia G. Rivera-Morales 2022. Serotyping . protocols.io https://dx.doi.org/10.17504/protocols.io.b4ysqxweVersion created by Lydia G. Rivera-Morales
Manuscript citation:
Palacios-Saucedo GdC, Rivera-Morales LG, Vázquez-Guillén JM, Caballero-Trejo A, Mellado-García MC, Flores-Flores AS, González-Navarro JA, Herrera-Rivera CG, Osuna-Rosales LE, Hernández-González JA, Vázquez-Juárez R, Barrón-Enríquez C, Valladares-Trujillo R, Treviño-Baez JD, Alonso-Téllez CA, Ramírez-Calvillo LD, Cerda-Flores RM, Ortiz-López R, Rivera-Alvarado M
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: February 11, 2022
Last Modified: March 17, 2022
Protocol Integer ID: 58098
Abstract
Serotyping of Streptococcus agalactiae isolates was carried out by ImmuLex® Strep-B Latex (Statens Serum Institut, Copenhagen, Denmark), classifying them in serotypes 1a,1b, II, III, IV, V, VII, VIII, or IX.
Use one colony from a 5-10% blood agar plate.
Add one drop (10l) of latex reagent on a glass slide.
Note
NOTE: Before using the latex reagent, the bottles should be at room
temperature. Remember to shake it.
Apply 10l phosphate buffer saline next to the drop of latex and suspend one
colony from the agar plate into the drop of saline.
Note
NOTE: Do not add too much colony material because that might give false
positives.
Mix the two drops
Note
NOTE: Use a different stick for each reaction.
Spread to cover the area of the circle.
Observe for agglutination within 5-10 seconds.
Note
NOTE: Any agglutination after 30 seconds is not a positive result.