This protocol outlines the steps followed to determine a potential "killer pore" in the area of failure of DMLS Ti6Al4V specimen. The methodology can be used more generally for positively identifying and quantifying small isolated pores in additively manufactured metals. In this case the focus was on analysis of a 4 mm region near the eventual fracture surface, to identify all pores in the vicinity of the "killer pore" - the one causing failure by fatigue tests. This protocol was developed for Ti6Al4V with 99.99% density (only small amount of porosity, very small pores only), tensile fatigue specimen round dogbone geometry with 5 mm gauge width.