May 12, 2020
Version 2
Sequencing open chromatin of single cell nuclei: snATAC-seq V.2
- Sebastian Preissl1,
- Xinxin Wang1,
- Bing Ren2
- 1Cellular and Molecular Medicine, University of California, San Diego;
- 2Ludwig Institute and Cellular and Molecular Medicine, University of California, San Diego
- BICCN / BICAN
Protocol Citation: Sebastian Preissl, Xinxin Wang, Bing Ren 2020. Sequencing open chromatin of single cell nuclei: snATAC-seq. protocols.io https://dx.doi.org/10.17504/protocols.io.4zzgx76
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 01, 2019
Last Modified: November 22, 2023
Protocol Integer ID: 25369
Abstract
Protocol for applying the TN5-based ATAC-seq method to the analysis of the chromatin of single cell nuclei isolated from brain tissue, as performed within the Center for Epigenomics of the Mouse Bain Atlas (CEMBA) and in coordination with the BRAIN Initiative Cell Census Network (BICCN).
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