The SensiFAST™ SYBR® & Fluorescein Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive real-time PCR. The kit has been validated on several Bio-Rad real-time PCR instruments. For ease-of-use and added convenience, SensiFAST SYBR® & Fluorescein is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers. The kit consists of a ready-to-use premix, only primers and template need to be added.