The protocol was creating adapting components from the manual from Bio-Rad for the analysis software ImageLab. Data analyzed using the protocol here described have appear in the following publications:Jim\u00e9nez-Soto LF, Haas R. The CagA toxin of Helicobacter pylori: abundant production but relatively low amount translocated. Sci Rep. 2016 Mar 17;6:23227. doi: 10.1038\/srep23227. PubMed PMID: 26983895; PubMed Central PMCID: PMC4794710.and in the title 'Host cell resistance to CagA translocation is as variable as Helicobacter pylori'. Zeitler et al 2017 accepted in Matters journal.