Aug 05, 2020
SEM imaging of Bacteria
- 1domnic colvin
- UM-DAE Centre for Excellence in Basic Sciences
Document Citation: avinash.kale 2020. SEM imaging of Bacteria. protocols.io https://dx.doi.org/10.17504/protocols.io.bjdjki4n
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: August 05, 2020
Last Modified: August 05, 2020
Document Integer ID: 40075
SEM imaging of Bacteria
The surface morphologies of the identified bacteria were imaged using Scanning Electron Microscopy (SEM) using ZEISS Ultra-field Emission SEM and ZEISS Environmental SEM at Tata Institute for Fundamental Research (TIFR), Mumbai, India. In brief, the sample were prepared by fixing then using 3% glutaraldehyde, followed by phosphate buffer washings. Subsequently, samples were subjected to ethanol dehydration and were thoroughly dried prior to the specimen mounting on the SEM grids at room temperature. (22)(23).