Apr 22, 2024
Self-made chrome alum gelatin coated slides
- Sonja Fritzsche1
- 1Max-Delbrueck-Center
Protocol Citation: Sonja Fritzsche 2024. Self-made chrome alum gelatin coated slides. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl49kkogo5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 22, 2024
Last Modified: April 22, 2024
Protocol Integer ID: 98589
Abstract
This is step-wise protocol to coating microscopy slides with chrom alum gelatin for better tissue adherence.
Materials
- Chromium(III) potassium sulphate dodecahydrate (#3535.3 Carl Roth)Carl RothCatalog #3535.3
- Gelatine from porcine skin Merck MilliporeSigma (Sigma-Aldrich)Catalog #48722
Prepare chrome alum gelatin solution
Prepare chrome alum gelatin solution
Heat up 200 mL ddH2O to 50 °C on hot plate using a magnetic stirrer.
Add 1 g gelatin gradually until completely dissolved to prevent formation of clumps. Solution has to be clear.
Let solution cool down to room temperature and add 0.1 g Chrome potassium sulfate until completely dissolved.
Either proceed immediately or store the solution for a few days @ 4 °C . If stored, let the solution warm-up to room temperature
before use.
Slide coating
Slide coating
10m 10s
Pour prepared chrome alum gelatin solution in a staining dish preheated at 60 °C .
Incubate to-be-treated slides in 70 % (v/v) Ethanol for 00:10:00 to clean them.
10m
Rinse in distilled water, dip at least 3 times to remove alcohol.
Dip the slides 5 times in the solution, 00:00:10 each.
10s
Leave the slides to drain for a few minutes onto a filter paper.
Dry the treated slides in an oven at 37 °C over night.
Store the slides in a dry box and protect from dust.
Protocol references
The protocol is adapted based on the following published protocols: