Select five plots as standard: 1 (weakest) - 5 (strongest), then other plots will be scored based on the similar with which standard plot.Seedling vigor is based on the size of seedling, greenness, uniformityScore each plot for seeding vigor was phenotyped by plotSeedling vigor was phenotyped every other day for six timesSeedling vigor phenotyping was started after 15 days of planting or 10 days after emergence