Nov 02, 2023

Public workspaceSDS-PAGE

DOI
https://dx.doi.org/10.17504/protocols.io.4r3l22er4l1y/v1
  • Alexander Röntgen1
  • 1University of Cambridge
Alexander Röntgen
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DOI: https://dx.doi.org/10.17504/protocols.io.4r3l22er4l1y/v1
Protocol Citation: Alexander Röntgen 2023. SDS-PAGE. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l22er4l1y/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 02, 2023
Last Modified: November 02, 2023
Protocol Integer ID: 90291
Keywords: page protocol, sd, page, protocol
Abstract
Protocol for running an SDS-PAGE.
Materials
  • NuPAGE Sample Buffer 4X Concentrate (ThermoFisher Scientific)
  • Heating block
  • XCell SureLockTM Mini-Cell (ThermoFisher Scientific)
  • 4-12% Bis-Tris gels (ThermoFisher Scientific)
  • NuPAGE MES SDS Running Buffer (ThermoFisher Scientific)
  • SeeBlue Plus2 Pre-stained Protein Standard (ThermoFisher Scientific)
  • Instant Blue Protein stain (Abcam)
  • ChemiDoc MP Imaging system (Bio-Rad)
Troubleshooting
Sample preparation
Dilute NuPAGE Sample Buffer 4X Concentrate into protein samples 


Heat for 10 min at 95 °C 
Gel
Run gel at ~150 V for 35 min
Incubate in staining solution until bands appear
Image using ChemiDoc MP Imaging system