Dec 15, 2022

Scott's Tap Water Substitute

This protocol is a draft, published without a DOI.
  • 1UC Davis
joshdm M
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Protocol Citation: joshdm 2022. Scott's Tap Water Substitute. protocols.io https://dx.doi.org/
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 15, 2022
Last Modified: December 15, 2022
Protocol  Integer ID: 74001
Keywords: bluing agent, water
Abstract
Used as a bluing agent in H&E staining
Protocol materials
Magnesium Sulfate HeptahydrateFisher ScientificCatalog #M63-500
Sodium BicarbonateMerck MilliporeSigma (Sigma-Aldrich)Catalog #s-5761
Measure and mix salts
weigh 20 g of Magnesium Sulfate HeptahydrateFisher ScientificCatalog #M63-500

weigh 2 g of Sodium BicarbonateMerck MilliporeSigma (Sigma-Aldrich)Catalog #s-5761

Mix in ddWater until completely dissolved
store at room temperature
8.3 to 8.7