Feb 03, 2026

Saturation Genome Editing V.2

DOI
https://dx.doi.org/10.17504/protocols.io.rm7vzjyd4lx1/v2
  • 1University of Washington, Department of Genome Sciences, Brotman Baty Institute
Nahum Smith
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DOI: https://dx.doi.org/10.17504/protocols.io.rm7vzjyd4lx1/v2
Collection Citation: Nahum Smith, Silvia Casadei 2026. Saturation Genome Editing. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzjyd4lx1/v2Version created by Nahum Smith
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 02, 2026
Last Modified: February 03, 2026
Collection  Integer ID: 242459
Keywords: saturation genome editing workflow, saturation genome editing this collection, saturation genome editing, workflow
Funders Acknowledgements:
NHGRI (IGVF)
Grant ID: HG011969
Abstract
This collection details the saturation genome editing workflow.
Guidelines
Data Analysis
  • Demultiplex samples from Illumina output directory using BCLConvert software.
Files
Protocol
Name
Design
Version 1
Created by
Image of Nahum Smith, University of Washington
Nahum SmithUniversity of Washington
Protocol
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Guide Cloning
Version 1
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Image of Nahum Smith, University of Washington
Nahum SmithUniversity of Washington
Protocol
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Library Cloning
Version 1
Created by
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Nahum SmithUniversity of Washington
Protocol
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Staining for FACS Sorting of Live Cells
Version 1
Created by
Image of Nahum Smith, University of Washington
Nahum SmithUniversity of Washington
Protocol
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Tissue culture
Version 1
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Image of Nahum Smith, University of Washington
Nahum SmithUniversity of Washington
Protocol
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Pre-Sequencing
Version 2
Created by
Image of Nahum Smith, University of Washington
Nahum SmithUniversity of Washington