This protocol was used to enrich for SARS-CoV2 sequencing reads from a confirmed COVID-19 swab sample and attain the full genome using an iSeq100. By using a spiked primer approach with 73 primers spanning the entire SARS-CoV2 genome, we were able to get an average of 15x genome coverage on an iSeq100 with 1.8 million paired end-reads. Here we overview all the steps, from sample extraction, library preparation with a spiked primer enrichment step, and sequencing on an iSeq100. The collaborative effort involved the Manning Lab, NIAID in Phnom Penh, Cambodia, Institut Pasteur Cambodge, Cambodia Ministry of Health, the Chan Zuckerberg Biohub, and the Chan Zuckerberg Initiative.This approach was developed based on the work from Deng et al, Nature Microbiology, January 13, 2020. https://www.nature.com/articles/s41564-019-0637-9