Sep 03, 2025

Public workspaceSanchiz Calvo et al., 2025

  • María Sanchiz Calvo1
  • 1KU Leuven
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Collection CitationMaría Sanchiz Calvo 2025. Sanchiz Calvo et al., 2025. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv5krb6v1b/v1
Manuscript citation:
Sanchiz-Calvo M, Coccia E, Cawthorne C, et al. Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits. Acta Neuropathol. 2025;150(1):5. Published 2025 Jul 17. doi:10.1007/s00401-025-02908-0
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: August 26, 2025
Last Modified: September 03, 2025
Collection Integer ID: 225505
Keywords: ASAPCRN, ATP10B, loss of the lysosomal lipid flippase atp10b, lysosomal lipid flippase atp10b, progressive dopaminergic neurodegeneration, parkinsonian motor deficit
Funders Acknowledgements:
ASAP
Grant ID: 024297
ASAP
Grant ID: 000458
Fonds wetenschappelijk onderzoek
Grant ID: I000321N
Abstract
all protocols related to 'Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits'; 10.1007/s00401-025-02908-0
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Files
Protocol
Name
Forkv2 RNAscope
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
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ForkFixation and Immunostaining protocol
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
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ForkiPSC Midbrain differentiation in 96 well plates
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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ForkImmunofluorescence staining, vibratome sections
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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Western blot for tissue extract
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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ForkImmunofluorescence staining ASE, vibratome sections
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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ForkCatalepsy test in rats
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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ForkElevated body swing test in rats
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
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ForkCylinder test in rats
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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ForkOpen field test in rats
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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ForkRotarod test in rats
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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Micro-PET CT procedures for brain imaging of rats
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
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Western blot for tissue extract
Version 1
, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
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Western blot protocol for detecting ATP10B in mouse/rat brain
Version 1
, KU Leuven
Eduard BenteaKU Leuven