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Collection CitationMaría Sanchiz Calvo 2025. Sanchiz Calvo et al., 2025. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv5krb6v1b/v1
Manuscript citation:
Sanchiz-Calvo M, Coccia E, Cawthorne C, et al. Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits. Acta Neuropathol. 2025;150(1):5. Published 2025 Jul 17. doi:10.1007/s00401-025-02908-0
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: August 26, 2025
Last Modified: September 03, 2025
Collection  Integer ID: 225505
Keywords: ASAPCRN, ATP10B, loss of the lysosomal lipid flippase atp10b, lysosomal lipid flippase atp10b, progressive dopaminergic neurodegeneration, parkinsonian motor deficit
Funders Acknowledgements:
ASAP
Grant ID: 024297
ASAP
Grant ID: 000458
Fonds wetenschappelijk onderzoek
Grant ID: I000321N
Abstract
all protocols related to 'Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits'; 10.1007/s00401-025-02908-0
Files
Protocol
Name
v2 RNAscope
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Fixation and Immunostaining protocol
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
iPSC Midbrain differentiation in 96 well plates
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Immunofluorescence staining, vibratome sections
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Western blot for tissue extract
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Immunofluorescence staining ASE, vibratome sections
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Catalepsy test in rats
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Elevated body swing test in rats
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Cylinder test in rats
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Open field test in rats
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Rotarod test in rats
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Micro-PET CT procedures for brain imaging of rats
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Western blot for tissue extract
Version 1
Created by
Image of María Sanchiz Calvo, KU Leuven
María Sanchiz CalvoKU Leuven
Protocol
Name
Western blot protocol for detecting ATP10B in mouse/rat brain
Version 1
Created by
Image of Eduard Bentea, KU Leuven
Eduard BenteaKU Leuven