Jan 22, 2024

Public workspaceSamuel Lab Media and Buffers

This collection is a draft, published without a DOI.
  • 1Baylor College of Medicine
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Collection CitationAdrien Assie, Buck Samuel 2024. Samuel Lab Media and Buffers. protocols.io https://protocols.io/view/samuel-lab-media-and-buffers-zyxf7xn
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 10, 2019
Last Modified: January 22, 2024
Collection Integer ID: 22263
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

Most of the protocols in this collection are derived from the wormbook protocol collection
Abstract
This is a collection of protocol for the preparation of standard media used in the Samuel Lab
Materials
1 M CaCl2 (Filter Sterilize)
5 mg/mL Cholesterol in 200 Proof Ethanol (Store at 4°C)
*500 mM EDTA, pH 8.0
5 M KOH
1 M MgSO­­4 (Autoclave)
5 M NaOH
*1 M Potassium Citrate Buffer, pH 6.0
*1 M Potassium Phosphate Buffer, pH 6.0
*Trace Metals Solution
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

Most of the protocols in this collection are derived from the wormbook protocol collection
Files
Protocol
Icon representing the file 0.5M EDTA, pH 8.0
Name
0.5M EDTA, pH 8.0
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file 1M Potassium Phosphate Buffer, pH 6.0
Name
1M Potassium Phosphate Buffer, pH 6.0
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file 1M Potassium Citrate Buffer, pH 6.0
Name
1M Potassium Citrate Buffer, pH 6.0
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file M9 Medium
Name
M9 Medium
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file LB borth or agar
Name
LB borth or agar
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file NGM Agar
Name
NGM Agar
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file Mannitol Agar
Name
Mannitol Agar
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file Worm Freezing Buffer
Name
Worm Freezing Buffer
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file Trace Metals Solution
Name
Trace Metals Solution
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file S-Basal Medium
Name
S-Basal Medium
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine
Protocol
Icon representing the file S-Complete Medium
Name
S-Complete Medium
Version 1
, Baylor College of Medicine
Adrien AssieBaylor College of Medicine