Collection Citation: Adrien Assie, Buck Samuel 2024. Samuel Lab Media and Buffers. protocols.io https://dx.doi.org/
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 10, 2019
Last Modified: January 22, 2024
Collection Integer ID: 22263
Keywords: samuel lab media, preparation of standard media, samuel lab, standard media, preparation, buffer
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
Most of the protocols in this collection are derived from the wormbook protocol collection
Abstract
This is a collection of protocol for the preparation of standard media used in the Samuel Lab
Materials
1 M CaCl2 (Filter Sterilize)
5 mg/mL Cholesterol in 200 Proof Ethanol (Store at 4°C)
*500 mM EDTA, pH 8.0
5 M KOH
1 M MgSO4 (Autoclave)
5 M NaOH
*1 M Potassium Citrate Buffer, pH 6.0
*1 M Potassium Phosphate Buffer, pH 6.0
*Trace Metals Solution
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
Most of the protocols in this collection are derived from the wormbook protocol collection
