May 18, 2020
Version 2
Sampling strategies and sample preservation Acropora V.2
- 1Pennsylvania State University
- Coral Bleaching RCN protocols
- Astrangia
Protocol Citation: Iliana Baums 2020. Sampling strategies and sample preservation Acropora. protocols.io https://dx.doi.org/10.17504/protocols.io.bec8jazw
Manuscript citation:
Kitchen SA, Von Kuster G, Vasquez Kuntz KL, Reich HG, Miller W, Griffin S, Fogarty ND, Baums IB (2020) STAGdb: a 30K SNP genotyping array and Science Gateway for Acropora corals and their dinoflagellate symbionts. bioRxiv 10.1101/2020.01.21.914424:2020.2001.2021.914424. doi:10.1101/2020.01.21.914424.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 30, 2020
Last Modified: May 18, 2020
Protocol Integer ID: 34944
Keywords: genetic sampling, coral, genotyping, preservation, coral SNPchip, coral genotpying array,
Abstract
Protocol describes sampling strategies, perservation and shipping of Acropora samples for
Applied Biosystems™ Axiom™ Coral Genotyping Array – 550961 (384f)
Applied Biosystems™ Axiom™ Coral Genotyping Array – 550962 (Mini 96)
Attachments
Guidelines
Please consult the word document for details on how to sample coral tissue for use of
Applied Biosystems™ Axiom™ Coral Genotyping Array – 550961 (384f)
Applied Biosystems™ Axiom™ Coral Genotyping Array – 550962 (Mini 96)
Materials
Please contact these providers to process your samples using the Axiom array:
Dawn Waltman
Genotyping Technical Sales Specialist
Microarray, Open Array and Agriseq
ThermoFisher Scientific
3450 Central Expressway/Santa Clara, CA 95051
(Cell) 410-688-0723
or
Farhad Ghavami, Ph.D.
Chief Scientific Officer (CSO)
Agrigenomics
Eurofins BioDiagnostics Inc.
507 Highland Dr.
River Falls, WI 54022
Tel: (715) 629-1938
Fax: (715)426-0251
E-mail: farhadghavami@eurofinsus.com
Safety warnings
Do not attempt to ship samples preserved in ethanol without proper training
Ethanol is flammable
Corals are listed under CITES and require permits for sampling and exporting
The Pennsylvania State University does not receive royalties or any other form of payment from Applied Biosystems for commercializing the genotyping array.
Before start
Please contact these providers to process your samples using the Axiom array:
Dawn Waltman
Genotyping Technical Sales Specialist
Microarray, Open Array and Agriseq
ThermoFisher Scientific
3450 Central Expressway/Santa Clara, CA 95051
(Cell) 410-688-0723
or
Farhad Ghavami, Ph.D.
Chief Scientific Officer (CSO)
Agrigenomics
Eurofins BioDiagnostics Inc.
507 Highland Dr.
River Falls, WI 54022
Tel: (715) 629-1938
Fax: (715)426-0251
E-mail: farhadghavami@eurofinsus.com