Sep 08, 2020

Public workspaceSampleholder preparation

DOI
dx.doi.org/10.17504/protocols.io.bky4kxyw
  • 1ETHZ - ETH Zurich
  • XPRIZE Rapid Covid Testing
philippe.bechtold
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DOI: dx.doi.org/10.17504/protocols.io.bky4kxyw
Protocol Citation: philippe.bechtold 2020. Sampleholder preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.bky4kxyw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 07, 2020
Last Modified: September 08, 2020
Protocol Integer ID: 41724
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The sampleholder contains 9 wells that are preloaded with an RT-qPCR Mastermix as follows:
Wells 1 to 3 contain probes and primers for the N1 target, wells 2 to 6 contain probes and primers for the N2 target, wells 7 to 9 contain probes and primers for the Human RP target. Wells 7,8,9 contain the respective positive control plasmid
The mastermix preloaded contains the following: Amount2.5 µL Geneon RT-PCR Mastermix (2x) ,r Concentration500 micromolar (µM) of each Primer , Concentration125 micromolar (µM) TaqMan Probe , Concentration0.05 µg/µL BSA ,Concentration1 millimolar (mM) MgSO4 filled up to Amount5 µL with RNAse free water per well

The sample holders with the mix are lyophilized with the following procedure: Freezing in Temperature-80 °C for Duration02:00:00 , transfer into precooled lyophilizer at Temperature-55 °C for Duration03:00:00 at least followed by transfer into a dissicator for heating up under vacuum.

Sample holders are vacuum packaged for further use.