Feb 08, 2025
Sample preparation for PIV in organ-on-a-chip device
- 1University of Latvia
Protocol Citation: Viesturs Sints 2025. Sample preparation for PIV in organ-on-a-chip device. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1dme2vr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 07, 2025
Last Modified: February 08, 2025
Protocol Integer ID: 119793
Keywords: sample preparation for piv, piv measurement, preparation of sample, sample preparation, piv, chip device, ooc device, sample, chip device the protocol, preparation, optical system
Abstract
The protocol describes preparation of samples to be used in PIV measurements performed in OOC devices. Keep in mind that the substances and variables may change significantly depending on the optical system and OOC device used.
Prepare 1 mL flow substance
Take 50 µL of tracer particle solution (example: 1 μm Nile Red; must be chosen for the laser used in PIV and the optical system) and add to flow substance
Shake for 00:00:05 in a lab tube shaker
5s
Place for 00:30:00 in an ultrasonic bath
30m
The sample is now prepared for PIV measurements. It cannot be reliably expected to retain colloidal stability for more than 01:00:00