Sep 11, 2025
Sample Preparation Correlative XPCT - Histology
- Jonas Franz1,
- Katja Schulz1,
- Christine Stadelmann1
- 1University Medical Center Göttingen, Neuropathology
Protocol Citation: Jonas Franz, Katja Schulz, Christine Stadelmann 2025. Sample Preparation Correlative XPCT - Histology. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl495b8go5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 01, 2024
Last Modified: September 11, 2025
Protocol Integer ID: 102641
Keywords: sample preparation correlative xpct, preparation of correlative histological analysis, histology this protocol, correlative histological analysis, histology, xpct, placement of sample, preparation, sample
Funders Acknowledgements:
Aligning Science Across Parkinson’s ASAP
Grant ID: 020625
Abstract
This protocol describes the placement of samples for XPCT and preparation of correlative histological analysis after the measurement.
Troubleshooting
XPCT-Sample Preparation
Selection of ROI in a FFPE tissue block and punch biopsy with 1 mm diameter to be placed in Kapton tube
perform XPCT measurement as described here:
Reembedding of FFPE tissue punch
perform at RT this delicate preparation step
hold Kapton tube with sample inside using fine forceps
push sample carefully out of the Kapton tube
prefill casting mold with warm paraffin and place it at RT
place sample with tissue facing the bottom in liquid paraffin during the curing process of paraffin
finally place cassette holder on top and place the new paraffin block on a cold plate
proceed to regular slicing using a microtome