Oct 22, 2021
S. elongatus stock revival
- 1IISER Pune
- iGEM IISER Pune India 2021
Protocol Citation: Akashdutta 2021. S. elongatus stock revival. protocols.io https://dx.doi.org/10.17504/protocols.io.bzcxp2xn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: October 22, 2021
Last Modified: October 22, 2021
Protocol Integer ID: 54391
Abstract
Stocks are more permanent methods of storing bacteria than plates. These stocks can be recovered by pouring them into small volumes of BG-11 and growing under low light levels. As the culture grows, it can be passaged into higher volumes and appropriate antibiotics can be added.
Add your stock to 2 mL BG-11 medium in a test tube and grow it overnight.
Note
Do not add any antibiotics
Transfer this to a 50 mL flask and make up the culture to 10 mL in volume.
Once it reaches a lime green colour (#32CD32), add a fresh medium to make the culture up to 50 mL culture in a 150 mL
Further, make this culture up to 100 mL in a 250 mL flask once it returns to lime green.