Sep 02, 2020
RT-LAMP Reaction
- Noah Toppings1
- 1University of Calgary
- XPRIZE Rapid Covid Testing
Protocol Citation: Noah Toppings 2020. RT-LAMP Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.bkp7kvrn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 02, 2020
Last Modified: September 02, 2020
Protocol Integer ID: 41439
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Add 9.5 µL extracted RNA + 15 µL resuspension buffer + 0.5 µL of dye to a lyophilized reaction pellet.
Mix reaction by pipetting.
Add 30 µL mineral oil on top of reaction.
Spin down the strip of tubes.
Incubate at 61 ºC for 45 minutes.
Visualize reactions under a transilluminator.